RAPD-PCR – still a suitable Method for Genetically Underexplored Species?

Metacercaria Identification Using Hat-rapd Pcr Method

The infection dynamics of metacercariae were assessed in cyprinoid fish (cyprinoids) from Chiang Mai and Sakon Nakhon Provinces, Thailand, during October 2008 to September 2009. The samples were collected during 3 seasons from rivers and local markets. Metacercarial infection was determined by acidpepsin digestion and confirmed using HAT-RAPD PCR method. Thirteen and 16 species of cyprinoids we...

Mycotoxins and Nuclear Receptors: A Still Underexplored Issue

Mycotoxins are fungal secondary metabolites that can be found in food commodities worldwide. They exert a wide range of adverse effects towards humans and animals. Although toxicological studies have addressed these food contaminants over decades, their mode of actions as well as their synergistic effects are still to be deeply clarified. Among the toxicological targets, nuclear receptors have ...

High-throughput DNA extraction method suitable for PCR.

PCR has become one of the most popular techniques in functional genomics. Projects in both forward and reverse genetics routinely require PCR amplification of thousands of samples. Processing samples to extract DNA of sufficient purity for PCR is often a limiting step. We have developed a simple 96-well plate-based high-throughput DNA extraction method that is applicable to many plant species. ...

Thymus: still an underexplored organ in medical practice

Saltgrass, a potential future landscaping plant and a suitable species for desert regions: A review

Continuous desertification of arable lands mandates use of low quality/ saline water for irrigation, especially in regions experiencing water shortage. Using low quality/ saline water for irrigation imposes more stress on plants that are already under stress in these regions. Thus, a logical solution will be to find a salt/ drought-tolerant plant species that will survive/sustain under such str...